Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording
نویسندگان
چکیده
منابع مشابه
Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording.
Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput o...
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Reid R. Harrison, Ilya Kolb, Suhasa B. Kodandaramaiah, Alexander A. Chubykin, Aimei Yang, Mark F. Bear, Edward S. Boyden,* and Craig R. Forest* Intan Technologies, Los Angeles, California; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia; Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts; McGovern Institute, Mass...
متن کاملPatch clamp techniques for single channel and whole-cell recording
The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
متن کاملClosed-Loop Real-Time Imaging Enables Fully Automated Cell-Targeted Patch-Clamp Neural Recording In Vivo.
Targeted patch-clamp recording is a powerful method for characterizing visually identified cells in intact neural circuits, but it requires skill to perform. We previously developed an algorithm that automates "blind" patching in vivo, but full automation of visually guided, targeted in vivo patching has not been demonstrated, with currently available approaches requiring human intervention to ...
متن کاملPatch and Whole-Cell Recording
Last time we considered at some length the sources of random noise due to the thermal motion of ions and electrons. Now we turn to the practical matter of dealing with the pickup of nonrandom electrical signals, such as interference from the 60 Hz power lines or from a computer monitor. There are two main principles to consider: electrostatic pickup through unshielded surfaces, and magnetic pic...
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ژورنال
عنوان ژورنال: Journal of Neurophysiology
سال: 2015
ISSN: 0022-3077,1522-1598
DOI: 10.1152/jn.00629.2014